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biological source
human eye
growth mode
Semi-adherent
karyotype
Not specified
morphology
Grape like clusters of round cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Retinoblastoma
Cell Line Description
Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.
Culture Medium
Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol
Subculture Routine
Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.
Other Notes
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Certificates of Analysis (COA)
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