C9336

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): CAT Antibody - Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit, Cat Antibody
• MDL number: MFCD01092314
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 26 kDa
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene; western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Chloramphenicol Acetyl Transferases (CATs) shows conservation and differences in their amino acid sequences. CAT is encode by cat gene and exists as monomer and later assemble into a trimer.

Specificity

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD).

Immunogen

bacterial chloramphenicol acetyl transferase (CAT).

Application

Anti-Chloramphenicol Acetyl Transferase (CAT) has been used in

• immunoblotting
• indirect immunofluorescence
• immunofluorescence microscopy

Biochem/physiol Actions

Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable