C8482
Protein Kinase A catalytic subunit human
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Synonym(s):
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
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About This Item
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recombinant
expressed in E. coli
Quality Level
Assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
>1000 units/mg protein
mol wt
43.5 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PRKACA(5566)
Biochem/physiol Actions
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
Physical form
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Analysis Note
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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