C5736
Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse
clone 15C10, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Anti-CANP, Anti-CANP1, Anti-CANPL1, Anti-SPG76, Anti-muCANP, Anti-muCL
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About This Item
conjugate:
unconjugated
application:
ELISA (i)
IP
WB CL
IP
WB CL
clone:
15C10, monoclonal
species reactivity:
bovine, mouse, rat, human
citations:
10
technique(s):
immunoprecipitation (IP): 2-5 μL (native and denaturing conditions)
indirect ELISA: suitable
western blot (chemiluminescent): 1:1,000
indirect ELISA: suitable
western blot (chemiluminescent): 1:1,000
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15C10, monoclonal
form
buffered aqueous glycerol solution
species reactivity
bovine, mouse, rat, human
technique(s)
immunoprecipitation (IP): 2-5 μL (native and denaturing conditions)
indirect ELISA: suitable
western blot (chemiluminescent): 1:1,000
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CAPN1(823) , CAPNS1(826)
mouse ... Capn1(12333) , Capns1(12336)
rat ... Capn1(29153) , Capns1(29156)
General description
Calpain-1 (CAPN1), an intracellular, calcium-dependent cysteine protease, has four domains, such as, the N-terminal anchor helix region, the CysPc protease domain, the C2 domain-like domain and the penta-EF-hand domain (PEF). CAPN1 codes for μ-calpain protein. CAPN1 gene is abundantly expressed in the central nervous system (CNS). This gene is located on human chromosome 11q13.1.
Specificity
Specifically recognizes the 80 kDa subunit of μ-calpain as well as two smaller proteins that are presumed to be degradation products.
Immunogen
purified human μ-calpain from placenta (calpain-I, protein kinase-C activating factor, E.C. 3.4.22.17).
Application
Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse has been used in:
- western blotting
- co-immunoprecipitation
- immunofluorescence
- immunofluorescence staining
Biochem/physiol Actions
Calpain-1 (CAPN1) plays a key role in microtubular regulation, cerebellar development, synaptic plasticity, synaptic restructuring, axon maturation and maintenance. CAPN1 activation is essential to provide a neuro-protective role in central nervous system (CNS). Mutations on CAPN1 gene results in spastic paraplegia 76 (SPG7), a complicated form of hereditary spastic paraplegia (HSP).
Physical form
Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, and 3 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Monica Averna et al.
Biochimica et biophysica acta, 1812(12), 1649-1657 (2011-10-11)
We are here reporting that in peripheral blood mononuclear cells (PBMC) of patients homozygous for F508del-CFTR the calpain-calpastatin system undergoes a profound alteration. In fact, calpain basal activity, almost undetectable in control PBMC, becomes measurable at a significant extent in
Christian Giordano et al.
PloS one, 10(6), e0131068-e0131068 (2015-06-25)
Short-term intermittent hypoxia (IH) is common in patients with acute respiratory disorders. Although prolonged exposure to hypoxia induces atrophy and increased fatigability of skeletal muscle, the response to short-term IH is less well known. We hypothesized that the diaphragm and
Wei Cui et al.
Cell & bioscience, 5, 36-36 (2015-07-07)
Under inflammatory conditions or during tumor progression macrophages acquire distinct phenotypes, with factors of the microenvironment such as hypoxia and transforming growth factor β (TGFβ) shaping their functional plasticity. TGFβ is among the factors causing alternative macrophage activation, which contributes
Yadira Bastián et al.
Reproduction (Cambridge, England), 140(5), 673-684 (2010-08-19)
Research on fertilization in mammalian species has revealed that Ca(2+) is an important player in biochemical and physiological events enabling the sperm to penetrate the oocyte. Ca(2+) is a signal transducer that particularly mediates capacitation and acrosome reaction (AR). Before
Dina Aweida et al.
EMBO reports, 23(12), e55175-e55175 (2022-10-25)
Maintenance of desmin intermediate filaments (IF) is vital for muscle plasticity and function, and their perturbed integrity due to accelerated loss or aggregation causes atrophy and myopathies. Calpain-1-mediated disassembly of ubiquitinated desmin IF is a prerequisite for desmin loss, myofibril
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