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B9429

Sigma-Aldrich

Anti-Bcl-xL antibody, Mouse monoclonal

~2 mg/mL, clone 2H12, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD01864113
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2H12, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

species reactivity

rat, mouse, human

concentration

~2 mg/mL

technique(s)

microarray: suitable
western blot: 25-100 μg/mL using human epidermoid carcinoma A431 cell extract

isotype

IgG2a

UniProt accession no.

Q07817

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BCL2L1(598)
mouse ... Bcl2l1(12048)
rat ... Bcl2l1(24888)

Specificity

The antibody reacts with Bcl-xL protein, but not with Bcl-xS.

Immunogen

synthetic peptide corresponding to amino acids 3-14 shared by murine and human Bcl-xL, conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
086M4793V
014M4778
121M4847
028K4775
017K4804

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Liu, X.-H., et al.
Proceedings of the National Academy of Sciences of the USA, 96, 9563-9563 (1997)
G Basañez et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(10), 5492-5497 (1999-05-13)
Release of proteins through the outer mitochondrial membrane can be a critical step in apoptosis, and the localization of apoptosis-regulating Bcl-2 family members there suggests they control this process. We used planar phospholipid membranes to test the effect of full-length
K G Wolter et al.
The Journal of cell biology, 139(5), 1281-1292 (1998-01-07)
Bax, a member of the Bcl-2 protein family, accelerates apoptosis by an unknown mechanism. Bax has been recently reported to be an integral membrane protein associated with organelles or bound to organelles by Bcl-2 or a soluble protein found in
Rhonda J Staversky et al.
American journal of respiratory cell and molecular biology, 43(3), 376-385 (2009-11-03)
Recent studies indicate that the antiapoptotic Bcl-X(L), one of five isoforms expressed by the Bcl-X gene, protects a variety of cell lines exposed to hyperoxia. However, its role in lung development and protection against oxidative stress in vivo is not
Y T Hsu et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(8), 3668-3672 (1997-04-15)
Bcl-2, Bcl-X(L), and Bax are members of the Bcl-2 family that play key roles in the regulation of apoptosis. These proteins are believed to be membrane bound and their ability to undergo both homodimerization and heterodimerization has been proposed to
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