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A5463

Sigma-Aldrich

Anti-Acetylated Proteins antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD03454689
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, chicken, bovine

concentration

~0.5 mg/mL

technique(s)

indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells
microarray: suitable
western blot: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate

shipped in

dry ice

storage temp.

−20°C

General description

Protein acetylation on lysine is a reversible protein modification that regulates protein functions. Histone acetylation at N-epsilon-lysine residues can destabilize the nucleosome structure and render DNA more accessible to transcription factors. Protein acetylation controls the function of transcription factors such as p53, GATA-1, E2F, TFIIEb, TFIIF, and EKLF.
Staining of histone H4 in immunoblotting is specifically inhibited with acetylated histone H4 immunizing peptide (human, amino acids 1-20 with N-epsilon-acetylated lysines at positions 5, 8, 12, and 16). No inhibition with the non-acetylated histone H4 peptide (human, amino acids 1-20) is observed.
Reacts with several acetylated proteins (e.g., acetylated histone H4 [12 kDa], acetylated H1 and H2b and acetylated BSA). Does not recognize acetylated histone H3.

Immunogen

synthetic acetylated peptide corresponding to the N-terminus of human histone H4 (amino acids 1-20, with N-ε-acetylated lysines at positions 5,8,12 and 16) conjugated to KLH.

Application

Rabbit anti-acetylated protein antibody can be used for microarrays, indirect immunofluorescence (1:1,000) and western blot (1:1,000) assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Stéphane Bourque et al.
The New phytologist, 192(1), 127-139 (2011-06-10)
• Plant resistance to pathogen attack is often associated with a localized programmed cell death called hypersensitive response (HR). How this cell death is controlled remains largely unknown. • Upon treatment with cryptogein, an elicitor of tobacco defence and cell
J Boyes et al.
Nature, 396(6711), 594-598 (1998-12-22)
Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA. A breakthrough in understanding how this acetylation is mediated was the discovery that
L Liu et al.
Molecular and cellular biology, 19(2), 1202-1209 (1999-01-16)
The p53 tumor suppressor protein is a sequence-specific transcription factor that modulates the response of cells to DNA damage. Recent studies suggest that full transcriptional activity of p53 requires the coactivators CREB binding protein (CBP)/p300 and PCAF. These coactivators interact

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