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Key Documents

68528

Sigma-Aldrich

Peroxidase from Bjerkandera adusta

≥2.8 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204

biological source

fungus (Bjerkandera adusta)

Quality Level

form

powder

specific activity

≥2.8 U/mg

storage temp.

−20°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Application

Peroxidase, product 68528, is isolated from Bjerkandera adusta. Peroxidases from B. adusta, a white rot fungus, is used to degrade synthetic dyes . Peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Peroxidases are used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Peroxidase is commonly used to determine amounts of glucose and peroxides in solution.

Biochem/physiol Actions

The transformation of industrial dyes by manganese peroxidases from Bjerkandera adusta is a manganese-independent reaction .

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmol Mn2+ to Mn3+ per minute at pH 4.5 and 25°C (in the presence of H2O2).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

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A Heinfling et al.
Applied and environmental microbiology, 64(8), 2788-2793 (1998-08-04)
We investigated the transformation of six industrial azo and phthalocyanine dyes by ligninolytic peroxidases from Bjerkandera adusta and other white rot fungi. The dyes were not oxidized or were oxidized very little by Phanerochaete chrysosporium manganese peroxidase (MnP) or by
G M Cook et al.
Microbiology (Reading, England), 144 ( Pt 12), 3297-3308 (1999-01-12)
Escherichia coli produces an extracellular factor that inhibits the aerobic growth of Cyd- mutants, defective in the synthesis or assembly of the cytochrome bd-type quinol oxidase. This paper shows that such a factor is the iron-chelating siderophore enterochelin. Mutants in
Y P Chau et al.
Acta anatomica, 153(2), 135-144 (1995-01-01)
Vascular permeability in various rat sympathetic ganglia, including superior cervical ganglia, thoracic ganglia and the celiac-mesenteric ganglia (CMG) complex, was investigated by using lanthanum and horseradish peroxidase (HRP) as tracers with special attention to the neuronal and small granule-containing (SGC)
G A Lambert et al.
Cephalalgia : an international journal of headache, 11(2), 75-85 (1991-05-01)
The superior sagittal sinus, middle meningeal artery or superficial temporal artery was stimulated electrically in anaesthetized cats. Field potential recordings were used to locate areas of maximum responses in the upper cervical cord, which were then further examined for responsive
Wen Liu et al.
Biochemical pharmacology, 85(10), 1504-1512 (2013-03-20)
NLRP3 inflammasome has been reported to be associated with various kinds of immunological diseases including colitis. However, there are few drug candidates targeting inflammasomes for the treatment of colitis. In the present study, we aimed at examining the effect of

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