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Key Documents

51309

Sigma-Aldrich

TRIS borate-EDTA buffer concentrate, long run

for Northern and Southern blotting, solution

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About This Item

MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioReagent

form

solution

quality

10× concentrate
ready-to-use solution

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

storage temp.

room temp

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Components

The TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

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Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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