Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

47140

Sigma-Aldrich

4-Fluoro-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

NBD-F

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
29270-56-2
Molecular Weight:
183.10
Beilstein:
1077571
MDL number:
MFCD00010196
UNSPSC Code:
12352100
PubChem Substance ID:
329757296
NACRES:
NA.32

product line

BioReagent

Assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Dansyl chloride suitable for fluorescence, BioReagent, ≥99.0% (HPLC)

Sigma-Aldrich

39220

Dansyl chloride

Bryan R Fonslow et al.
Analytical chemistry, 80(9), 3182-3189 (2008-03-21)
The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants.
Chanda Ciriacks Klinker et al.
Analytical chemistry, 79(22), 8747-8754 (2007-10-13)
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for
Zsolt Wagner et al.
Electrophoresis, 32(20), 2816-2822 (2011-10-20)
Three different fluorescent tags have been compared for the quantitative analysis of aspartate and glutamate in brain microdialysate samples. Separation conditions have been optimized to achieve short analysis time using reversed polarity separation in coated capillary. Method validation has revealed
Hua-Ming Tseng et al.
Analytical and bioanalytical chemistry, 388(2), 433-439 (2007-03-21)
A rapid and sensitive capillary electrophoresis (CE) method has been developed for profiling organic metabolites containing amine functional groups in mammalian biofluids. Metabolites containing an amine group were derivatized with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazol (NBD-F), separated by micellar electrokinetic chromatography (MEKC), and detected
Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service