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406OA-05A

Sigma-Aldrich

Human Osteoblasts-Osteoarthritis: HOb-OA, adult

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human bone (osteoarthritic)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

osteoblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

arthritis

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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Human Osteoblasts-Osteoarthritis, HOb-OA, are isolated from bone of patients with osteoarthritis (OA) and provide an excellent model system to study the patophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.

Characterization: Positive for bone mineralization.

Cell Line Origin

Bone

Application

model system to study the pathophisiology of osteoarthritis, matrix mineralization, effects of inflammatory mediators, bone morphogenic proteins, bone remodeling and metabolism

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HOb-OA Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

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Xiangxiang Sun et al.
International journal of biological sciences, 16(6), 994-1009 (2020-03-07)
Osteoarthritis (OA) is a common type of arthritis. Chronic inflammation is an important contributor to the pathogenesis of OA. The maturation and secretion of proinflammatory cytokines are controlled by inflammasomes, especially NLRP1 (NLR Family Pyrin Domain Containing 1) and NLRP3.

Protocols

Human Osteoblasts Osteoarthritis (HOb-OA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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