22178
Cellulase from Aspergillus niger
powder, off-white, ~0.8 U/mg
Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase
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About This Item
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biological source
Aspergillus niger
Quality Level
form
powder
specific activity
~0.8 U/mg
color
off-white
storage temp.
2-8°C
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General description
Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.
Application
Cellulase from Aspergillus niger has been used:
- in the preparation of the standardized solution to determine absolute cellulase levels
- in phosphate-citric acid buffer for activity assay
- in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed
Biochem/physiol Actions
Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
Unit Definition
1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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M.A. Sawsan et al.
Zentralblatt Fur Mikrobiologie, 143, 55-55 (1988)
G. Okada
Methods in Enzymology, 160, 259-259 (1988)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%
P. Toussaint et al.
Entropie, 23 (136), 8-8 (1987)
Helena Fernandes et al.
Bioresource technology, 281, 277-285 (2019-03-03)
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed
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