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Key Documents

116-500

Sigma-Aldrich

Fibroblast Growth Medium (500 ml)

Synonym(s):

Primary Cell Culture Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

Quality Level

100

shelf life

6 mo.

manufacturer/tradename

Cell Applications, Inc

shipped in

wet ice

storage temp.

2-8°C

General description

All-in-one ready-to-use media

Application

Fibroblast Growth Medium (500 ml) has been used to culture human fibroblast cells. It has also been used to culture adult human primary normal gingival fibroblast cells.

Quality

Each lot was tested for the ability to support plating, spreading and proliferation

Preparation Note

contained in protocol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tung-Nien Hsu et al.
Cancers, 11(12) (2019-12-01)
FAT atypical cadherin 1 (FAT1) regulates cell-cell adhesion and extracellular matrix architecture, while acting as tumor suppressor or oncogene, context-dependently. Despite implication of FAT1 in several malignancies, its role in oral squamous cell carcinoma (OSCC) remains unclear. Herein, we document

Protocols

Human Dermal Fibroblasts (HDF) Culture Protocol

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

Generating organotypic 3D skin cultures as an in-vitro model of the human epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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