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S0751

Sigma-Aldrich

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

Synonym(s):

Monosodium dihydrogen orthophosphate, Monosodium phosphate, Sodium dihydrogen phosphate

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About This Item

Linear Formula:
NaH2PO4
CAS Number:
Molecular Weight:
119.98
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
38070208
PubChem Substance ID:
NACRES:
NA.21

Quality Level

product line

ReagentPlus®

Assay

≥99.0%

form

powder or crystals

technique(s)

DNA extraction: suitable
electrophoresis: suitable
immunocytochemistry: suitable

pH

4.0-4.5 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 100 mg/mL, clear, colorless

application(s)

detection

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.

Application

Sodium phosphate monobasic has been used:
  • as a reagent for virus transfection and fiber implantation in mouse
  • in phosphate buffer for immunocytochemistry and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)
  • as a buffer for cycling assay
  • in automated DNA extraction method
  • as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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In vitro Brainstem-spinal Cord Preparation from Newborn Rat
Le Gal JP, et al.
The Journal of Neuroscience (2016)
Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
Kanamori KS, et al.
Bio-protocol, 8(14) (2018)
Dictionary of Food ingredients (2001)
Mild blast injury produces acute changes in basal intracellular calcium levels and activity patterns in mouse hippocampal neurons
Hansen KR, et al.
Journal of Neurotrauma, 35(13), 1523-1536 (2018)
Automated and manual methods of DNA extraction for Aspergillus fumigatus and Rhizopus oryzae analyzed by quantitative real-time PCR
Francesconi A, et al.
Journal of Clinical Microbiology, 46(6), 1978-1984 (2008)

Protocols

To standardize a procedure for the enzymatic assay of Urease, from Jack Beans

Protocol for the enzymatic Trypsinogen Assay

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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