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Girard′s reagent T

for HPLC derivatization, LiChropur™, 99.0-101.0% (AT)

Synonym(s):

(Carboxymethyl)trimethylammonium chloride hydrazide, (Hydrazinocarbonylmethyl)trimethylammonium chloride, Acethydrazide trimethylammonium chloride

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About This Item

Linear Formula:
[(CH3)3N+CH2CONHNH2]Cl-
CAS Number:
123-46-6
Molecular Weight:
167.64
Beilstein:
3727163
EC Number:
204-629-3
MDL number:
MFCD00012009
UNSPSC Code:
12000000
PubChem Substance ID:
329769730
NACRES:
NA.22

grade

for HPLC derivatization

Quality Level

100

Assay

99.0-101.0% (AT)

form

crystals

quality

LiChropur™

technique(s)

HPLC: suitable

mp

188-192 °C (dec.) (lit.)

SMILES string

[Cl-].C[N+](C)(C)CC(=O)NN

InChI

1S/C5H13N3O.ClH/c1-8(2,3)4-5(9)7-6;/h4,6H2,1-3H3;1H

InChI key

YSULOORXQBDPCU-UHFFFAOYSA-N

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Application

Girard′s reagent T may be used as a derivatizing reagent for the determination of 5-Formyl-2′-deoxyuridine in cellular DNA samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique. Reducing saccharides can be derivatized by Girard′s reagent T and this can be analyzed by matrix-assisted laser desorption/ionization coupled to time of flight mass spectrometry (MALDI-TOF-MS) technique.

Features and Benefits

  • Girard′s reagent T is a chemical derivatization reagent for LC-MS.
  • It has carbonyl functional group possessing high proton (cation) affinity that stabilizes a positive charge.       
  • It is commonly used in the derivatization of nucleosides.        
  • Derivatization reactions in mass spectrometry are used to improve ionization efficiency.
  • Derivatization can enhance precise quantitative analysis for profiling of relatively small analyte molecules, particularly in metabolomics.

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG6488
BCCF6003
BCCB5401
BCBZ5762
BCBW1699

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Oksana Lavrynenko et al.
Journal of lipid research, 54(8), 2265-2272 (2013-07-12)
Ecdysteroids are potent developmental regulators that control molting, reproduction, and stress response in arthropods. In developing larvae, picogram quantities of individual ecdysteroids and their conjugated forms are present along with milligrams of structural and energy storage lipids. To enhance the
Applications of MALDI-TOF Spectroscopy (2014)
Haizheng Hong et al.
Analytical chemistry, 79(1), 322-326 (2006-12-30)
Nucleoside 5-formyl-2'-deoxyuridine (FodU) is a major thymidine lesion generated by reactive oxygen species. In vitro and in vivo replication studies revealed that FodU can be mutagenic. A reliable and sensitive quantification method is, therefore, important for assessing the biological implications

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