Skip to Content
Merck
All Photos(1)

Key Documents

75482

Sigma-Aldrich

Gram′s decolorizer solution

for Gram staining

Synonym(s):

Acetone ethanol mixture

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
37348-17-7
MDL number:
MFCD00240361
UNSPSC Code:
12171500
NACRES:
MA.02

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

refractive index

n20/D 1.361

density

0.80 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

General description

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolorizer solution is a mixture of ethanol and acetone.

Application

Gram′s decolorizer solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolorizer dissolves the lipid layer from the gram-negative cells.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Supplementary Hazards

EUH066

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCG4116
BCCD7425
BCCC5370
BCCB6462
BCBZ9634

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens, their Properties and their Detection

An article regarding the Role of Clostridium perfringens and their detection, identification, and differentiation from Sigma-Aldrich.com

On the Trail of Campylobacter

On the Trail of Campylobacter - The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis. However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness. Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar fl agella

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service