66720
Methylene Blue hydrate
purum p.a., for microscopy (Bact., Bot., Hist., Vit.), ≥97.0% (calc. based on dry substance, AT)
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Recommended Products
grade
for microscopy (Bact., Bot., Hist., Vit.)
purum p.a.
Quality Level
Assay
≥97.0% (calc. based on dry substance, AT)
SMILES string
O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
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General description
Methylene Blue (MB) is a basic histological dye.
Application
Methylene Blue hydrate has been used:
- in the preparation of methylene blue (MB) solution to stain zymograms
- to label the injection site in the tissue slices
- to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss
Biochem/physiol Actions
Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors.
Other Notes
may contain up to 22% water
On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis
Signal Word
Warning
Hazard Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Bio-protocol, 5(3), e1687-e1687 (2015)
Attenuation of noise-induced hearing loss using methylene blue
Park JS, et al.
Cell Death & Disease, 5(4), e1200-e1200 (2014)
S Caglio et al.
Electrophoresis, 14(10), 997-1003 (1993-10-01)
The efficiency of a novel method of photopolymerization, based on photoinitiating the reaction with methylene blue (MB), in presence of a redox couple (sodium toluenesulfinate and diphenyliodonium chloride), vs, the conventional persulfate--N,N,N',N'-tetramethylethylenediamine redox couple was investigated as a function of
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