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556416

Sigma-Aldrich

New Methylene Blue N

Dye content ~70 %

Synonym(s):

Basic Blue 24, Methylene Blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S
CAS Number:
1934-16-3
Molecular Weight:
347.91
Colour Index Number:
52030
EC Number:
217-696-9
MDL number:
MFCD00135878
UNSPSC Code:
12171500
PubChem Substance ID:
24879556
NACRES:
NA.47

form

powder, crystals or chunks

composition

Dye content, ~70%

technique(s)

microbe id | staining: suitable

mp

190 °C (dec.) (lit.)

λmax

590 nm
632 nm (2nd)

εmax

≥29000 at 626-634 nm in water
≥30500 at 586-594 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C

InChI

1S/C18H22N3S.ClH/c1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;/h7-10,19-20H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

NZYCYASKVWSANA-UHFFFAOYSA-M

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Application

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.

Biochem/physiol Actions

New methylene blue N can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).

Features and Benefits

Solvent soluble cationic thiazine dye

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN9708
SHBL0614
MKAA3423V
MKAA3423
16203EO

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Diego A Portela et al.
Veterinary anaesthesia and analgesia, 39(4), 398-408 (2012-05-31)
To describe the landmarks and methodology to approach the thoracic paravertebral space in dogs; to evaluate if intercostal muscular response could be evoked by a nerve-stimulator; to radiographically assess the distribution pattern of a radio-opaque contrast medium after thoracic paravertebral
Jennifer L Lansdowne et al.
American journal of veterinary research, 66(8), 1324-1329 (2005-09-22)
To determine the relationship between epidural cranial migration and injectate volume of an isotonic solution containing dye in laterally recumbent foal cadavers and evaluate the cranial migration and dermatome analgesia of an epidural dye solution during conditions of laparoscopy in
M Shaffer et al.
Pediatric pathology, 13(5), 591-595 (1993-09-01)
Although reticulocyte counts can be reliably performed for up to 48 h after storage in EDTA, it is unclear whether this is applicable to the pediatric age group. In order to evaluate this, manual reticulocyte counts were performed on 20
S K Lai et al.
Clinical and laboratory haematology, 21(4), 261-264 (1999-12-03)
Hb-H disease is a form of alpha-thalassemia commonly found in south-east Asia. In this condition, numerous Hb-H bodies are found in erythrocytes using reticulocyte staining preparations. This is the first study addressing the possible interference of Hb-H inclusions in automated
Turgeon ML
Linne & Ringsrud's Clinical Laboratory Science: The Basics and Routine Techniques (2014)
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