11796895001
Roche
DIG Easy Hyb™ Granules
powder, suitable for hybridization
Synonym(s):
DIG system
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About This Item
UNSPSC Code:
41105326
Agency
suitable for
Quality Level
form
powder
usage
sufficient for 6 x 100 mL
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
technique(s)
hybridization: suitable
color
white
pH
7,0
solubility
water: soluble
suitability
suitable for nucleic acid hybridization
greener alternative category
storage temp.
20-25°C
General description
DIG Easy Hyb™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.
Features and Benefits
- DIG Easy Hyb™ is a convenient ready-to-use hybridization buffer, saving time and effort.
- DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.
Preparation Note
Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
DIG Easy Hyb is a trademark of Roche
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting
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