SF006
ESGRO Complete Accutase
The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
Synonym(s):
Stem Cell Tested Accutase
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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Quality Level
form
liquid
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
shipped in
dry ice
General description
ESGRO Complete™ Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.
Application
Cell Detachment:
1. Thaw Accutase® to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
1. Thaw Accutase® to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
Physical form
Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
Storage and Stability
Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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PloS one, 3(4), e2025-e2025 (2008-04-24)
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Stem cell reports, 3(1), 169-184 (2014-07-30)
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Hsi-Lung Hsieh et al.
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