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NA70

Sigma-Aldrich

Anti-p14ARF (Ab-2) Mouse mAb (14PO2)

liquid, clone 14PO2, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

14PO2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CDKN2A(1029)

General description

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
This Anti-p14ARF (Ab-2) Mouse mAb (14PO2) is validated for use in Immnunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p14ARF (Ab-2).

Immunogen

Human
recombinant, human p14ARF (p16β)

Application

Immnunoblotting (2-4 µg/ml)

Immunofluorescence (0.5 µg/ml)

Immunoprecipitation (2 µg/mg lysate)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Normal sheep serum
Positive Control
HeLa cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Bates, S., et al. 1998. Nature395, 124.

Legal Information

Sold under license of U.S. Patent 6,482,929.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stephen L Lessnick et al.
Cancer cell, 1(4), 393-401 (2002-06-28)
Ewing's sarcoma is associated with a fusion between the EWS and FLI1 genes, forming an EWS/FLI fusion protein. We developed a system for the identification of cooperative mutations in this tumor through expression of EWS/FLI in primary human fibroblasts. Gene
Chongwei Xie et al.
FEBS letters, 586(4), 435-441 (2012-01-31)
ARF is the second most commonly inactivated tumor suppressor behind p53. It has been implicated in the control of cell proliferation, cell senescence, and tumor suppression. However, the detailed mechanism underlying the transcriptional control of ARF remains largely unknown. Here

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