MABN1539
Anti-Serotonin Receptor 1B Antibody, clone 1C2.1
clone 1C2.1, from mouse
Synonym(s):
5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C2.1, monoclonal
species reactivity
human, rat, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HTR1B(3351)
General description
5-hydroxytryptamine receptor 1B (UniProt P28222; also known as 5-HT-1B, 5-HT-1D-beta, 5-HT1B, S12, Serotonin 1D beta receptor, Serotonin receptor 1B) is encoded by the HTR1B (also known as HTR1DB) gene (Gene ID 3351) in human. The 5-HT receptors are grouped according to their antagonist susceptibilities and their affinities for 5-HT. The 5-HT1 receptors are a subfamily of 5-HT receptors that were originally classified according to their inhibition of adenylyl cyclase, degree of sequence similarity and their overlapping pharmacological specificities. The subfamily has five different receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F that can couple to Gi/Go and mediate inhibitory neurotransmission. 5HT-1B receptor belong to the family of G-protein coupled receptors that are distributed in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen. 5-HT-1B also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide. Ligand binding causes a conformation change in the receptor that triggers signaling via G-proteins, which modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling via 5-HT-1B inhibits adenylate cyclase activity. 5-HT1B receptor is known to be involved in thermoregulation, respiration, appetite control, sexual behavior, and aggression.
5-HT-1B receptor are therapeutic targets for antimigraine drugs and for treatment of bipolar disorders, autism, and anti-aggressive agents.
5-HT-1B receptor are therapeutic targets for antimigraine drugs and for treatment of bipolar disorders, autism, and anti-aggressive agents.
Specificity
Clone 1C2.1 targets an epitope within the third cytoplasmic domain of human, mouse, and rat serotonin receptor 1B.
Immunogen
GST-tagged recombinant fragment corresponding to the thrid cytoplasmic domain of human serotonin receptor 1B.
Application
Anti-Serotonin Receptor 1B, clone 1C2.1 Antibody, Cat. No. MABN1539, is a highly specific mouse monoclonal antibody that targets serotonin receptor 1B and has been tested in Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected serotonin receptor 1B in human (cerebellum and cerebral cortex) and rat (cerebral cortex and medulla) brain tissue sections.
Western Blotting Analysis: 1 µg/mL from a representative lot detected serotonin receptor 1B in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 1 µg/mL from a representative lot detected serotonin receptor 1B in 10 µg of mouse brain tissue lysate.
Research Category
Neuroscience
Neuroscience
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 1B in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 1B in 10 µg of rat brain tissue lysate.
Target description
~43 kDa observed. 43.57/43.08/43.16 kDa (human/mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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