MABE1896

Anti-JunB Antibody

• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 75-5, monoclonal
• mol wt: calculated mol wt 29.10 kDa; observed mol wt ~43 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgGκ
• UniProt accession no.: P09450
• shipped in: dry ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Junb(16477)

Description

General description

Transcription factor Jun-B (UniProt: P09450; also known as MyD21) is encoded by the Junb (also known as Jun-b) gene (Gene ID: 16477) in mouse. JunB is a member of the AP-1 transcription family that binds to the DNA sequence 5′-TGA[CG]TCA-3′ and is involved in regulating gene activity following the primary growth factor response. It can bind to DNA either as a homodimer or as a heterodimer with another member of the Jun/Fos family. JunB plays an important role in proliferation, differentiation, and death. With respect to cell cycle control, JunB is reported to have a dual function. It can serve as a cell division inhibitor and a tumor suppressor or can promote cell division. It is reported to diminish cell proliferation via transcriptional activation of p16 (INK4a). Its expression is shown to be very low in quiescent cells but can be rapidly induced by mitogenic stimuli during the G0/G1 transition. It induces proliferation, survival, drug resistance in multiple myeloma (MM) cells and silencing of JunB is reported to block MM cell proliferation in a murine xenograft model. It also protects MM cells against dexamethosome and bortezomib-induced cell death. JunB is also reported to regulate epidermal stem cells and sebaceous glands through balancing proliferation and differentiation of progenitors and by suppressing lineage infidelity. (Ref.: Singh, K., et al. (2018). Nat. Commun. 9; Article 3425; Fan, F., et al (2017). Leukemia. 31(7); 1570-1581; Farras, R., et al. (2008). Mol. Cell. Biol. 28(12); 4173-4187; Passegue, E., and Wagner, EF. (2000). EMBO J. 19(12); 2969-2979).

Specificity

Clone 75-5 is a mouse monoclonal antibody that detects human JunB. It targets an epitope within the N-terminal region.

Immunogen

GST-tagged recombinant fragment correspond to the first 75 amino acids from the N-terminal region of mouse JunB.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected JunB in HeLa cell lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected JunB in Western Blotting applications (Passegue, E., et. al. (2000). EMBO J. 19(12):2969-79; Farras, R., et. al. (2008). Mol Cell Biol. 28(12):4173-87; Gomard, T., et. al. (2010). PLoS One. 5(3):e9585).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected JunB in HeLa cell line.

Immunocytochemistry Analysis: A representative lot detected JunB in Immunocytochemistry applications (Bakiri, L., et. al. (2000). EMBO J. 19(9):2056-68).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-JunB, clone 75-5, Cat. No. MABE1896, is a mouse monoclonal antibody that detects JunB and is tested for use in Immunocytochemistry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1