MAB4301

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

clone MC-480, Chemicon^®, from mouse

• Synonym(s): SSEA-1, CD15, Lewis X, 3-FAL
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: saturated ammonium sulfate (SAS) precipitated
• antibody product type: primary antibodies
• clone: MC-480, monoclonal
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• input: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s); sample type: human embryonic stem cell(s)
• isotype: IgM
• NCBI accession no.: NM_002033.2
• UniProt accession no.: P22083
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... FUT4(2526)

Description

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Immunogen

F9 tetracarcinoma stem cells (X-irradiated).

Application

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.

Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Neural Stem Cells

Quality

Tested

Target description

220 kDa

Physical form

Format: Purified

Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
P19 mouse embryonic carcinoma cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable