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Key Documents

ABE596

Sigma-Aldrich

Anti-ZEB1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

UniProt accession no.

P37275

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ZEB1(6935)

General description

Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8), Negative regulator of IL2, and NIL-2-A zinc finger protein acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB1 .

Application

Detect ZEB1 using this rabbit polyclonal antibody, Anti-ZEB1 Antibody validated for use in western blotting, ICC & Immunofluorescence.
Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB1 in HeLa cells.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ZEB1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ZEB1 in 15 µg of HeLa cell lysate.

Target description

~130 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes two isoforms at ~124 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hong Chang et al.
Scientific reports, 6, 22312-22312 (2016-03-01)
MicroRNAs are small and non-coding RNA molecules with the master role in regulation of gene expression at post-transcriptional/translational levels. Many methods have been developed for microRNA loss-of-function study, such as antisense inhibitors and sponges; however, the robustness, specificity, and stability
Xin Yao et al.
Oncotarget, 8(42), 72235-72249 (2017-10-27)
The Transducin-like enhancer of split 1 (TLE1) corepressor protein is overexpressed in human lung tumors and is a putative lung-specific oncogene. However, the molecular mechanism underlying its oncogenic function remains to be delineated. Here, we report an important role of

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