ABC40
Anti-AIP1/Alix Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Programmed cell death 6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
mouse ... Pdcd6Ip(18571)
General description
Programmed cell death 6-interacting protein (UniProt: Q9WU78; also known as ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2) is encoded by the Pdcd6ip (also known as Aip1, Alix) gene (Gene ID: 18571) in murine species. AIP1 is a class E VPS cytoplasmic, ubiquitously expressed protein that is involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intra-lumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. AIP1 interacts with ALG-2 (apoptosis-linked gene-2) to regulate cell death. This interaction is strictly dependent on calcium. AIP1 is considered to be essential for the maintenance of fibroblast morphology and has been shown to play a role in the regulation of both apoptosis and cell proliferation. AIP1 is also known to regulate exosome biogenesis in concert with SDC1/4 and SDCBP. AIP1 can undergo phosphorylated on tyrosine residues by activated platelet-derived growth factor receptor-beta (PDGFRB).
Specificity
This polyclonal antibody specifically detects AIP1 in Jurkat cells. It is likely to react with isoforms 1, 2, and 3.
Immunogen
GST-tagged recombinant fragment corresponding to 479 amino acids from the C-terminal half of mouse AIP1/Alix.
Application
Detect Programmed cell death 6-interacting protein using this rabbit polyclonal Anti-AIP1/Alix, Cat. No. ABC40 that has been tested in Immunocytochemistry and Western blotting.
Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected AIP1/Alix in HeLa and NIH3T3 cells.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.
Target description
~95 kDa observed; 96.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 20 mM Tris-HCl, pH 7.2, 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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