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AB9094-I

Sigma-Aldrich

Anti-Sodium Pump Subunit alpha-2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2, Sodium Pump Subunit alpha-2, Anti-Sodium Pump (Na+ pump (Na+-K+-ATPase)) α 2 Isoform, Sodium/po

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_036637

UniProt accession no.

P06686

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATP1A2(477)
rat ... Atp1A2(24212)

General description

Sodium/potassium-transporting ATPase subunit alpha-2 (EC 3.6.3.9; UniProt P06686; also known as Na+/K+ -ATPase alpha 2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2) is encoded by the Atp1a2 (also known as RATATPA2) gene (Gene ID 24212) in rat. Mammalian Na+/K+ ATPase is a heteromultimeric complex composed of alpha, beta, and gamma subunits. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ across the plasma membrane. The alpha subunit contains ten transmembrane domains with both its N- and C-termini in the cytoplasm. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders.

Specificity

Reacts with Sodium Pump (Na+ pump (Na+-K+-ATPase)) alpha 2 isoform. The antibody does not cross react with other Na+-K+-ATPase alpha isoforms.

Immunogen

Epitope: Second cytoplasmic domain
KLH-conjugated linear peptide corresponding to the second cytoplasmic domain of rat Sodium Pump Subunit alpha-2.

Application

Detect Sodium Pump Subunit alpha-2 using this Anti-Sodium Pump Subunit alpha-2 Antibody validated for use in Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.
Immunohistochemistry Analysis (IHC): A representative lot detected Sodium Pump Subunit alpha-2 in human (1:1,000) and rat (1:250) cerebellum tissue sections.

Immunoprecipitation Analysis (IP): 10 ug from a representative lot immunoprecipitated Sodium Pump Subunit alpha-2 in 500 µg of rat brain tissue lysate.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected Sodium Pump Subunit alpha-2 in 10 µg of rat brain tissue lysate.

Target description

~100 kDa observed

Linkage

Replaces: AB9094

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jeffrey X Xie et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 33(7), 1138-1149 (2018-01-03)
Recent studies have highlighted a critical role for CD40 in the pathogenesis of renal injury and fibrosis. However, little is currently understood about the regulation of CD40 in this setting. We use novel Na/K-ATPase cell lines and inhibitors in order
Declan Williams et al.
PloS one, 16(11), e0258682-e0258682 (2021-12-01)
The prion protein (PrP) is best known for its ability to cause fatal neurodegenerative diseases in humans and animals. Here, we revisited its molecular environment in the brain using a well-developed affinity-capture mass spectrometry workflow that offers robust relative quantitation.
Yuri M Poluektov et al.
Scientific reports, 9(1), 4872-4872 (2019-03-21)
Severe hypoxia leads to decline in cardiac contractility and induces arrhythmic events in part due to oxidative damage to cardiomyocyte proteins including ion transporters. This results in compromised handling of Ca2+ ions that trigger heart contractile machinery. Here, we demonstrate

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