AB3573
Anti-BCL-XL (pS62) Antibody
Chemicon®, from rabbit
Synonym(s):
Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rat (100% homologous), mouse (100% homologous)
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer62)
Gene Information
human ... BCL2L1(598)
General description
Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.
Specificity
Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react.
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat.
Application
Anti-BCL-XL (pS62) Antibody detects level of BCL-XL (pS62) & has been published & validated for use in WB.
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Quality
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62.
Linkage
Replaces: 04-432
Physical form
Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.
Storage and Stability
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Analysis Note
Control
SK-BR-3 cells treated with vinblastine.
SK-BR-3 cells treated with vinblastine.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Anti-HLA class I antibody-mediated activation of the PI3K/Akt signaling pathway and induction of Bcl-2 and Bcl-xL expression in endothelial cells.
Jin, Y.P., et al.
Human immunology, 65(4), 291-302 (2004)
Ochratoxin A induces apoptosis in human lymphocytes through down regulation of Bcl-xL.
Assaf, Hind, et al.
Toxicological Sciences, 79, 335-344 (2004)
Selective induction of apoptosis by PBOX-6 in leukemia cells occurs via the JNK dependent phosphorylation and inactivation of Bcl-2 and Bcl-XL.
Mc Gee, M.M., et al.
Journal of Pharmacology and Experimental Therapeutics, 310(3), 1084-1095 (2004)
Bcl-2 and Bcl-XL with nonpeptidic small-molecule antagonists.
Wang, S., et al.
Seminars in Oncology, 30(5 Suppl 16), 133-142 (2003)
K Sugiyama et al.
Cancer research, 59(17), 4406-4412 (1999-09-15)
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