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Key Documents

322133

Sigma-Aldrich

DSP, Water-Soluble

Synonym(s):

3,3-Dithio- bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP, 3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.24
Pricing and availability is not currently available.

Assay

≥94% (HPLC)

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

off-white to light tan

solubility

water: 6 mg/mL

shipped in

ambient

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
1.077331.093851.07729
Gel di silice 60 extra pure for column chromatography 0.063 - 0.200 mm (70 - 230 mesh ASTM)

1.07754

Gel di silice 60

Silice for column chromatography

1.07733

Silice

Gel di silice 60 (0,040-0,063 mm) for column chromatography (230-400 mesh ASTM)

1.09385

Gel di silice 60 (0,040-0,063 mm)

Silice for column chromatography

1.07729

Silice

matrix active group

Irregular-Shaped Silica Gel

matrix active group

Irregular-Shaped Silica Gel

matrix active group

-

matrix active group

Irregular-Shaped Silica Gel

material

silica gel (irregularly-shaped)

material

silica gel (irregularly-shaped)

material

silica gel (irregularly-shaped)

material

-

pore size

6 nm mean pore size

pore size

6 nm mean pore size

pore size

6 nm mean pore size

pore size

-

pH

7 (20 °C, 100 g/L in H2O, slurry)

pH

7 (20 °C, 100 g/L in H2O, slurry)

pH

7 (20 °C, 100 g/L in H2O, slurry)

pH

7 (20 °C, 100 g/L in H2O, slurry)

bp

2230 °C (lit.)

bp

2230 °C (lit.)

bp

2230 °C (lit.)

bp

2230 °C (lit.)

General description

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.

Warning

Toxicity: Irritant (B)

Reconstitution

Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.

Other Notes

Howard, A.D., et al. 1985. J. Biol. Chem.260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol.134, 518.
D′Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.
Yamada, H., et al. 1981. Biochem.20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem.4, 212.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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