208921

Calmodulin Kinase IINtide, Myristoylated

≥95% (HPLC), solid, CaMK II inhibitor, Calbiochem^®

• Synonym(s): Calmodulin Kinase IINtide, Myristoylated, Myr-N-GGGKRPPKLGQIGRAKRVVIEDDRIDDVLK-OH
• Empirical Formula (Hill Notation): C₂₅₆H₂₇₅N₄₇O₄₃
• Molecular Weight: 4698.21
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• product name: Calmodulin Kinase IINtide, Myristoylated, The myristoylated form of CaMK IINtide.
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

The myristoylated form of CaMK IINtide (Cat. No. 208920). The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

The myristoylated form of CaMK IINtide (Cat. No. 208920), a potent, specific inhibitor of Ca²⁺/Calmodulin kinase II (CaMK II) (IC₅₀ = 50 nM for total and Ca²⁺-independent CaMK II activity). The peptide sequence corresponds to the inhibitory domain of the CaMK II inhibitory protein, CaMK IIN. Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ = 100-400 nM). Shown to completely inhibit the phosphorylation of GluR1 fusion protein at a concentration of 1 µM. Does not inhibit CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Calmodulin-Dependent Protein Kinase (CaM Kinase)-2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 nM against cam Kinase-2

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Myr-N-Gly-Gly-Gly-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at-20°C.

Other Notes

Sodering, T.R., et al. 2001. J. Biol. Chem.276, 3719.
Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.
Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable