1.52352

Chromolith^® WP 300 Epoxy (monolithic) HPLC Columns

L × I.D. 25 mm × 2 mm HPLC Column

• Synonym(s): Chromolith^® WP 300 Epoxy 25 mm x 2 mm HPLC column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Chromolith^® WP 300 Epoxy, L × I.D. 25 mm × 2 mm HPLC column
• material: PEEK
• Quality Level: 100
• product line: Chromolith^®
• feature: endcapped: no
• packaging: pkg of 1 unit
• extent of labeling: 5.5% Carbon loading
• parameter: 2-60 °C temp. range; 200 bar pressure
• technique(s): HPLC: suitable
• column L × I.D.: 25 mm × 2 mm
• surface area: 120 m²/g
• matrix: monolithic silica
• matrix active group: Epoxy bonding phase
• pore size: 2 μm macroporosity; 30 nm mesoporosity
• operating pH range: 1.5-7.5
• separation technique: affinity

Description

General description

Chromolith^® WP 300 Epoxy columns are specially designed for the user-specific immobilization of ligands and their later application in HPLC. The unique bimodal pore structure of silica monoliths allows efficient coupling independent of molecule size. The wider mesopores also enable the use of proteins and antibodies as both ligand immobilized on the column, and later analyte separated by an immobilized column.
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.

Chromolith^® WP 300 Epoxy 25-2 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput
—Narrow column I.D. enables improved performance and improved MS-compatibility

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

Flyer: Create Your Own Column Chemistry - Chromolith^® WP 300 Epoxy 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany