1.50848

LiChrospher^® 100 RP-18 endcapped (10 µm) HPLC Columns

L x I.D. 250 mm x 4 mm, LiChroCART^® HPLC cartridge, manuCART^™ holder required for use

• Synonym(s): TM=["LiChrospher"] C18 TM=["LICHROCART"] cartridge for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: LiChrospher^® 100 RP-18 endcapped (10 µm) LiChroCART^® 250-4, suitable for HPLC
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: LiChrospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 21.6% Carbon loading
• parameter: 250 bar pressure; 60 °C max. temp.
• technique(s): HPLC: suitable; mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 25 cm × 4 mm
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 10 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

HPLC cartridge

LiChrospher^® RP-18 endcapped is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 endcapped reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 250-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application

• Integration of Solid-Phase Extraction and Reversed-Phase Chromatography in Single Protein-Coated Columns for Direct Injection of Bupivacaine in Human Serum: This study highlights the use of the LiChrosorb^® RP-8 HPLC column in a novel method integrating solid-phase extraction with reversed-phase chromatography. This approach significantly enhances the efficiency and accuracy of bupivacaine analysis in human serum, underscoring the column′s utility in clinical and pharmaceutical settings. The research demonstrates the column′s high performance in complex sample matrices, making it an invaluable tool for analytical chemists and researchers in drug monitoring and pharmacokinetics (Zarad et al., 2020).

Analysis Note

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Toluene): ≥ 20000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.5 - 3.0

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany