1.06177

TBE buffer

10 x pH 8.3 tris-borate-EDTA buffer

• Synonym(s): 10X TBE running buffer, Tris-Borate EDTA buffer
• UNSPSC Code: 41105319
• NACRES: NA.25

Properties

• form: liquid
• pH: 8.2-8.4 (20 °C in H₂O)
• density: 1.06 g/cm³ at 20 °C
• storage temp.: 15-25°C

Description

General description

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO^- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH₂ and Tris-NH₃⁺). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg²⁺ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.

Analysis Note

Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H360FD - H412
• Precautionary Statements: P202 - P261 - P273 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable