06-1119

Anti-TLR2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, toll/interleukin 1 receptor-like 4, CD282 antigen
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_003255
• UniProt accession no.: O60603
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TLR2(7097) ; mouse ... Tlr2(24088)

Description

General description

Toll-like receptor 2 (TLR2) is a single-pass type I membrane protein belonging to the Toll-like receptor family and is comprised of 14 LRR repeats and a single TIR domain. TLR2 interacts with LY96 or TLR1 to regulate innate immune response to bacterial lipoproteins and other components of the microbial cell wall. It induces the activation of NFκB, inflammatory response and cytokine secretion through interactions with MYD88 and TRAF6. It may also function as a promoter for apoptosis. High expression of TLR2 has been reported in blood leukocytes, specifically in bone marrow, lymph node, monocytes and spleen. It has also been observed in fetal liver and lung tissues. Modifications in gene expression have been implicated in an increased susceptibility to leprosy.

Specificity

This antibody recognizes the extracellular domain of TLR2.

Immunogen

Epitope: Extracellular Domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human TLR2.

Application

Anti-TLR2 Antibody is an antibody against TLR2 for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected TLR2 in normal human placenta tissue.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected TLR2 in 10 µg of RAW264.7 cell lysate.

Target description

~90 kDa observed.
Blocking without Tween is recommended to avoid the observance of additional bands.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
RAW264.7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable