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Key Documents

G006021

HPLC Analysis of Peptides on Proteomix® SCX-NP3, Volatile Buffer, ELSD and UV Detection

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)
mobile phase: (A) 5 mM ammonium acetate; (B) 50 mM ammonium acetate:acetonitrile (4:1, v:v)
gradient: 0 to 50% B in 20 min
flow rate: 0.6 mL/min
detector: ELSD (SofTA) and UV, 280 nm
injection: 5 μL
sample: 0.1 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma

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