Skip to Content
Merck
All Photos(1)

Key Documents

G005923

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Salmon on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

suitable for GC, application for SPE

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: Dispersive (QuEChERS)
sample/matrix: raw salmon spiked with PAHs at 100 ng/g
extraction process: add 25 mL acetonitrile and the contents of the Supel™ QuE Non-buffered tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm
extraction tube: Supel™ QuE Non-buffered tube, magnesium sulfate/sodium chloride (55294-U)
clean-up tube: Supel™ QuE Z-Sep (500 mg, part no. 55299-U), magnesium sulfate (450 mg, part no. 63135) (55299-U, 63135)
clean-up process: transfer 3 mL of acetonitrile layer, Supel QuE Z-Sep (500 mg) and MgSO4 (450 mg) into an extraction tube; (shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis)
column: SPB-608, 20 m x 0.18 mm I.D. x 0.18 μm
oven: 60 °C (1 min), 25 °C/min to 275 °C, 10 °C/min to 300 °C (13 min)
inj. temp.: 265 °C
detector: MS, SIM
MSD interface: 300 °C
carrier gas: helium, 1.5 mL/min, constant
injection: 1 μL, splitless (1 min)
liner: 4 mm I.D. FocusLiner™ with taper

suitability

application for SPE

application(s)

food and beverages

General description

Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034

Application

Extraction solvent: acetonitrile, 25 mL
Clean-up volume: 3 mL
Clearn-up tube: Supel™ QuE Z-Sep (500 mg, part no. 55299-U, magnesium sulfate (450 mg, part no. 63135)

Analysis Note

Sample pretreatment: Place 5 g ground sample into 50 mL centrifuge tube. Spike with 100 ng/g target PAHs and internal standards. Let sit 30 min. Add 5 mL water and homogenize.
This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of raw salmon for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Supel™ QuE Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested.

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SPB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Naphthalene
    2-Methylnaphthalene (β)
    1-Methylnaphthalene
    Biphenyl
    2,6-Dimethylnaphthalene
    Acenaphthylene
    Acenaphthene
    2,3,5-Trimethylnaphthalene
    Fluorene
    Dibenzothiophene
    Phenanthrene
    Anthracene
    1-Methylphenanthrene
    Fluoranthene
    Pyrene
    Retene
    Benzo[b]fluoranthene
    Benzo[k]fluoranthene
    Benzo[j]fluoranthene
    Benzo[e]pyrene
    Benzo[a]pyrene
    Perylene
    Dibenz[a,h]anthracene
    Benzo[ghi]perylene
    See All (24)

SPE tube or plate

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service