G005413
HPLC Analysis of Testosterone and 1-Testosterone on Ascentis® Express C18
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)
mobile phase: [A] water; [B] methanol; (20:80, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
sample: 50 μg/mL in 10:90 water:methanol
suitability
application for HPLC
application(s)
clinical
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
analytical column
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