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Key Documents

G001830

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid
gradient: 0 to 32% B in 20 min. (linear)
flow rate: 0.5 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 50 μg each Hb variants (A2, S and A0)

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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