ZRB2119

Anti-SPI-C Antibody, clone 1F10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Transcription factor Spi-C
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F10, recombinant monoclonal
• description: 1F10 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 29.18 kDa; observed mol wt ~32 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_689536
• UniProt accession no.: Q8N5J4
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SPIC(121599)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F10 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Transcription factor Spi-C. It targets an epitope within 18 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Transcription factor Spi-C.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SPI-C in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPI-C in Raji cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SPI-C in Jurkat cells.

Affinity Binding Assay: A representative lot of this antibody bound SPI-C peptide with a KD of 6.6 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Transcription factor Spi-C (UniProt: Q8N5J4; also known as Spi-C) is encoded by the SPIC gene (Gene ID: 121599) in human. Spi-C is an E26 transformation-specific transcription factor that is closely related to PU.1 and Spi-B. It binds to the PU-box, a purine-rich DNA sequence (5′-GAGGA[AT]-3′) that can act as a lymphoid-specific enhancer. Its expression is observed in fetal and adult spleen and lymph nodes and at lower levels in bone marrow and fetal liver.  Spi-C plays an important role in antibody-generating responses, B cell development, and red pulp macrophage generation. It is induced by heme- and NF-κB-dependent pathways in macrophages. In the B cell compartment, its activity is tightly regulated during development and differentiation. It is reported to promote the transition from large to small pre-B cells and regulate the generation of antibody-secreting cells.  In the myeloid lineage, Spi-C has an crucial role in generating splenic red pulp macrophages through a heme-dependent pathway. It is also involved in regulating the inflammatory profile of macrophages in response to NF-κB signaling where it promotes a protective, anti-inflammatory phenotype. Spi-B and Spi-C are reported to play opposing roles in secondary antibody responses and plasmablast differentiation. They both interact with intronic regions of the Bach2 locus to regulate transcription.  However, Spi-B activates Bach2 whereas Spi-C represses Bach2. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Raczkowski, HL., and DeKoter, RP. (2021). Mech. Dis. 13(5);  e1519; Laramée, A-S., et al. (2020). Front. Immunol. 11; 841).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable