ZRB1019
Anti-Fibrillin-1 Antibody, clone 6C19 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
FBN1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
clone
6C19, recombinant monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 312.3 kDa
observed mol wt ~35 kDa
species reactivity
human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 6C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Fibrillin-1. It targets an epitope within the N-terminal half.
Immunogen
GST/His-tagged recombinant fragment corresponding to 95 amino acids from the N-terminal half of human Fibrillin-1.
Application
Quality Control Testing
Western Blotting Analysis: A 1:1000 dilution of this antibody detected GST/His-tagged recombinant human Fibrillin-1.Tested applicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrillin-1 in human placenta tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Fibrillin 1 recombinant fragment with a KD of 1.7 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Western Blotting Analysis: A 1:1000 dilution of this antibody detected GST/His-tagged recombinant human Fibrillin-1.Tested applicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrillin-1 in human placenta tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Fibrillin 1 recombinant fragment with a KD of 1.7 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Fibrillin-1, clone 6C19 ZooMAb, Cat. No. ZRB1019, is a recombinant Rabbit monoclonal antibody that specifically targets Fibrillin-1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Fibrillin-1 (UniProt: P35555; also known as FBN1) is encoded by the FBN1 (also known as FBN) gene (Gene ID: 2200) in human. Fibrillin-1 is a structural component of the 10-12 nm diameter microfibrils of the extracellular matrix that conveys both structural and regulatory properties to load-bearing connective tissues. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Fibrillin-1 is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-44) that are cleaved off to generate the mature Fibrillin-1, which can be further cleaved by furin between residues 2731 and 2732 to produce asprosin (aa 2732-2871). Asprosin serves as a hormone that targets the liver to increase plasma glucose levels. It promotes glucose release from hepatocytes by activating protein kinase A. Fibrillin-1 is reported to play a role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMP), growth and differentiation factors, and latent TGF-beta binding proteins. It is also reported to regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels. It is also shown to negatively regulate osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11. Mutations in FBN1 gene are known to cause Marfan syndrome, a disorder of the connective tissue that affects skeletal, ocular, and cardiovascular systems. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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