ZRB1015
Anti-Irisin (FNDC5) Antibody, clone 2K10 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2K10, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 23.66 kDa (calculated for FNDC5. )
observed mol wt ~24 kDa
species reactivity
human
species reactivity (predicted by homology)
rat, mouse
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... FNDC5(252995)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 2K10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Fibronectin type III domain-containing protein 5 (FNDC5). It targets and epitope within the Irisin region.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the Irisin region of the extracellular domain of human Fibronectin type III domain-containing protein 5 (FNDC5).
Application
Anti-Irisin (FNDC5), clone 2K10 ZooMAb, Cat. No. ZRB1015, is a recombinant Rabbit monoclonal antibody that specifically targets Irisin (FNDC5) and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Irisin (FNDC5) in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Irisin (FNDC5) in human skeletal muscle cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Irisin (FNDC5) in human skeletal muscle tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound FNDC5 peptide with a KD of 4.5 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Irisin (FNDC5) in human skeletal muscle cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Irisin (FNDC5) in human skeletal muscle tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound FNDC5 peptide with a KD of 4.5 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Fibronectin type III domain-containing protein 5 (UniProt: Q8NAU1; also known as Fibronectin type III repeat-containing protein 2; FNDC5) is encoded by the FNDC5 (also known as FRCP2) gene (Gene ID: 252995) in human. FNDC5 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 32-152), a transmembrane domain (aa 153-173), and a cytoplasmic domain (aa 174-212). The mature FNDC5 can be further cleaved into Irisin (aa 32-143). FNDC5/Irisin is widely expressed and is considered to be a thermogenic, exercise-induced chemokine that is involved in the browning of adipose tissue. It is released mainly by the white subcutaneous adipose tissue and plays a regulatory role in conversion of white fat to brown fat and has a potential in curbing fat accumulation. Its levels are shown to be upregulated by exercise and it can be detected in the blood of individuals following endurance exercise. It has also been associated with reduction of pro-inflammatory cytokines while promoting secretion of anti-inflammatory cytokines in adipose tissue. Irisin is reported to facilitate glucose uptake by skeletal muscles and improve hepatic glucose and lipid metabolism thereby causing a positive effect on hyperlipidemia and hyperglycemia caused by obesity and metabolic syndrome. Lower levels of Irisin are reported in diabetic patients compared to normal subjects. Irisin stimulation of glucose uptake in muscle tissue involves the activation of AMPK pathway. (Ref.: Arhire LI., et al. (2019). Front Endocrinol.10; 524; Zhang, Y., et al. (2014). Diabetes. 63(2); 514-525).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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