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Key Documents

SAB5600213

Sigma-Aldrich

Anti-Human IgG4 antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM120, purified immunoglobulin

Synonym(s):

IGHG4, Ig gamma 4 chain C, Immunoglobulin gamma 4, Immunoglobulin heavy constant gamma 4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM120, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

ELISA: suitable (50ng/well - 200ng/well (for Capture)
0.05ug/mL - 0.2ug/mL (for Detection))

flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1-5 μg/mL
immunohistochemistry: 1—5 μg/mL
western blot: 0.5— 2 μg/mL

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Specificity

This antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Immunogen

Peptide corresponding to the hinge region of Human IgG4

Physical form

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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