SAB4702005

Monoclonal Anti-c-Myc tag Biotin produced in mouse

• Synonym(s): MRTL, MYCC, bHLH
• UNSPSC Code: 12352203

Properties

• conjugate: biotin conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: buffered aqueous solution
• species reactivity: ( Human), (Tagged fusion proteins in all species)
• concentration: 1 mg/ml
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P01106
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MYC(4609)

Description

General description

The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.

Specificity

The antibody 9E10 can be used to detect the c-Myc tag.

Immunogen

Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable