S9626

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

• Synonym(s): Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
• CAS Number: 9016-17-5
• Enzyme Commission number: 3.1.6.1 (BRENDA, IUBMB)
• EC Number: 232-772-1
• MDL number: MFCD00132399
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type H-1
• form: powder
• sulfatase activity: ≥10,000 units/g solid
• secondary activity: ≥300 unit/mg solid β-glucuronidase (at pH 5.0)
• mol wt: ~85 kDa by gel filtration
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Sulfatase from Helix pomatia has been used in:

• sample extraction
• sulfatase assays
• enzymatic hydrolysis
• extract preparation for glucosinolate analysis

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves