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R7644

Sigma-Aldrich

Transcript RNA Markers 0.28-6.6 kb

for RNA electrophoresis

Synonym(s):

0.28-6.6 kb Transcript RNA Markers, Transcript RNA markers for RNA electrophoresis

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

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General description

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) contains 9 RNA transcripts from 281 – 6,583 bases. Three μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Transcript RNAMarkers 0.28-6.6 kb are suitable for use as an RNA ladder for formaldehyde agarose gel electrophoresis. It is also suitable for the size determination of small RNA using native or denaturing agarose gel electrophoresis.

Components

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains a mixture of nine RNA transcripts, 281–6,583 bases
For optimal resolution, the recommended agarose gel concentration is 1.0%.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization.
Pinaud R, et al.
Nature Protocols, 3, 1370-1379 (2008)

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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