P7643

PIPES dipotassium salt

≥99% (titration)

• Synonym(s): 1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt
• Empirical Formula (Hill Notation): C₈H₁₆K₂N₂O₆S₂
• CAS Number: 108321-27-3
• Molecular Weight: 378.55
• MDL number: MFCD00069751
• UNSPSC Code: 12161700
• PubChem Substance ID: 24898738
• NACRES: NA.25

Properties

• Quality Level: 200
• Assay: ≥99% (titration)
• form: crystalline powder
• useful pH range: 6.1-7.5
• pKa (25 °C): 6.8
• solubility: water: 0.25 g/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• SMILES string: [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
• InChI key: BJQYFCAMUXGZFN-UHFFFAOYSA-L

Description

General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves