N1638

Nystatin Suspension

suspension, 10,000 unit/mL in DPBS, BioReagent, suitable for cell culture

• Synonym(s): Fungicidin, Mycostatin
• CAS Number: 1400-61-9
• UNSPSC Code: 12352207
• PubChem Substance ID: 24897511
• NACRES: NA.76

Properties

• product line: BioReagent
• Quality Level: 200
• form: suspension
• concentration: 10,000 unit/mL in DPBS
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: yellow
• antibiotic activity spectrum: fungi; yeast
• Mode of action: cell membrane | interferes
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: C[C@@H]1OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@]2(O)C[C@H](O)[C@H]([C@H](C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\CC\C=C\C=C\[C@H](C)[C@@H](O)[C@@H]1C)O2)C(O)=O
• InChI: 1S/C47H75NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-36(53)35(52)20-19-31(49)21-32(50)22-33(51)23-39(55)62-29(3)28(2)42(27)56/h5-6,8,10-18,27-38,40-44,46,49-54,56-58,61H,7,9,19-26,48H2,1-4H3,(H,59,60)/b6-5+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28+,29-,30+,31+,32+,33+,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• InChI key: VQOXZBDYSJBXMA-QEKUPDCNSA-N

Description

General description

Chemical structure: polyene

Application

Nystatin suspension has been used:

• to inhibit caveolin-mediated endocytosis
• as a component of Eagle′s minimum essential medium (EMEM)
• to enumerate lactic acid bacteria (LAB)
• in preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in toll-like receptor 2 (TLR2)-expressing THP1 cells.

This product is recommended for use in cell culture applications at 24 mL/L.

Biochem/physiol Actions

Nystatin is a polyene, broad-spectrum antibiotic, which modifies the microstructure of lipid rafts. It can sequester cholesterol present on the cell membrane of eukaryotes. Nystatin regulates immune responses through cytokine signaling, lymphocyte activation and pathogen recognition.

Caution

This product is stable at 37°C for three days.

Preparation Note

It is 0.1μ filtered and cell culture tested.

Other Notes

Nystatin suspension available in 20 mL and 100 mL pack size.
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable