MTOX1105
MRP3 Knockout HepaRG™ Cells
human female liver (hepatocarcinoma and hepatitis C)
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
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product name
MRP3 Knockout HepaRG™ Cells, 1 vial
biological source
human female liver (hepatocarcinoma and hepatitis C)
Quality Level
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... MRP3(8714)
General description
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MRP3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
Application
See technical bulletin for detailed protocols
Features and Benefits
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.
- Sigma′s HepaRG MRP3 Knockout (KO) allows investigations of drug-transporter interactions involving MRP3 in the liver.
Zinc finger nucleases (ZFN) mediated Knock-out of ABCC3 (MRP3) gene.
- The frame-shift mutation of ABCC3 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
Quality
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Legal Information
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Sigma-Aldrich presents an article on The Role of Liver Transporters in Drug-Drug Interactions
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