MAK168

Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests (Blue fluorescence)

• Synonym(s): High-Sensitivity Pyrophosphate Assay Kit
• EC Number: 200-664-3
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 200 fluorometric tests (Blue fluorescence)
• application(s): cosmetics; food and beverages
• detection method: fluorometric
• storage temp.: −20°C

Description

General description

Pyrophosphate (PP_i) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.

Suitability

The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.

Principle

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λ_ex =316/λ_em = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H318
• Precautionary Statements: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C