L8757
Lysing Enzymes from Rhizoctonia solani
crude powder
Synonym(s):
Kitalase
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About This Item
UNSPSC Code:
12352204
form
crude powder
storage temp.
2-8°C
Application
Lysing enzymes at a concentration of 1mg/ml can be used for preparation of protoplasts from Rhizoctonia solani.
Biochem/physiol Actions
Main enzymatic acitivity is β−(1-3)-glucanase, also reported to contain protease, pectinase, and amylase activities.
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Expression of a cephalosporin C esterase gene in Acremonium chrysogenum for the direct production of deacetylcephalosporin C
Basch, J., et al
Journal of Industrial Microbiology, 31(11), 531-539 (2004)
Structural characterization of a neutral antitumour β-D-glucan extracted with hot sodium hydroxide from cultured fruit bodies of Grifola frondosa.
Iino, K., et al
Carbohydrate Research, 141(1), 111-119 (1985)
Molecular characterization of a Stagonospora nodorum lipase gene LIP1
Feng, J., et al
Plant Pathology, 60(4), 698-708 (2011)
Tung-Hsen Liu et al.
New biotechnology, 27(1), 64-69 (2009-09-01)
Novozym 234 was the most frequently used enzyme for production of Rhizoctonia solani protoplasts. Since manufacture of this enzyme was discontinued in the late 1990s, a new procedure was developed by testing lytic enzymes from Sigma and by examining factors
D Tsuchiya et al.
Phytopathology, 91(4), 354-360 (2008-10-24)
ABSTRACT Cytological karyotypes with mitotic metaphase chromosomes were analyzed for Cochliobolus heterostrophus, C. carbonum, and C. sativus by the germ tube burst method (GTBM). Prior to karyotyping, procedures of GTBM suitable to Cochliobolus were established by examining several crucial conditions
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