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Key Documents

L023050

Sigma-Aldrich

DCA Deblock

configured for (ÄKTA® and OligoPilot®)

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About This Item

UNSPSC Code:
41116004

description

with Toluene

Quality Level

200

type

for DNA synthesis
for DNA synthesis

storage condition

dry at room temperature

concentration

42.2-51.6 g/L (DCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear

compatibility

configured for (ÄKTA® and OligoPilot®)

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General description

Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. This solution contains toluene / Dichloroactic Acid (97/3 v/v).

Application

DCA Deblock solution facilitates removal of the DMT that allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Preparation Note

Toluene / Dichloroacetic acid 97/3 (v/v)

Legal Information

OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

39.2 °F - closed cup

Flash Point(C)

4 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
HMBJ3578
HMBJ3518
HMBJ3420
HMBJ3421
HMBJ3415

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